PrimePCR™ Probe Assay: Clip3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0039978
Assay Design:   intron_spanning
Chromosome Location:   1:92105892-92109392question
Amplicon Length:   113
Splice Variants Targeted:   ENSRNOT00000025157 ENSRNOT00000060832 ENSRNOT00000001129 ENSRNOT00000075610 ENSRNOT00000046886 ENSRNOT00000044865 ENSRNOT00000041277 ENSRNOT00000006403 ENSRNOT00000066708 ENSRNOT00000022002 ENSRNOT00000012927 ENSRNOT00000003837 ENSRNOT00000010373 ENSRNOT00000009468 ENSRNOT00000074960 ENSRNOT00000038160 ENSRNOT00000028247 ENSRNOT00000072819 ENSRNOT00000017948 ENSRNOT00000023247

Gene Information

Description Not Available

Gene Symbol:   Clip3
Gene Name:   Protein Clip3; Similar to CLIP-170-related protein (Predicted)
Aliases:   Not Available
RefSeq:   NM_001107501
Ensembl:   ENSRNOG00000050104
Entrez:   308493
UniGene:   Rn.136605
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.220000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download