PrimePCR™ Probe Assay: Atp5g1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Atp5g1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Atp5g1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0038597
Assay Design:   intron_spanning
Chromosome Location:   10:83701431-83703383question
Amplicon Length:   177
Splice Variants Targeted:   ENSRNOT00000046318 ENSRNOT00000038207 ENSRNOT00000018982 ENSRNOT00000039102 ENSRNOT00000000607 ENSRNOT00000003951 ENSRNOT00000049325 ENSRNOT00000012996 ENSRNOT00000060708 ENSRNOT00000047210 ENSRNOT00000067771 ENSRNOT00000032359 ENSRNOT00000003837 ENSRNOT00000039336 ENSRNOT00000014158 ENSRNOT00000047380 ENSRNOT00000060943 ENSRNOT00000067509 ENSRNOT00000024348 ENSRNOT00000026156 ENSRNOT00000039417 ENSRNOT00000024900 ENSRNOT00000009815 ENSRNOT00000038993 ENSRNOT00000048175 ENSRNOT00000059942 ENSRNO

Gene Information

isoform of mitochondril ATP synthase subunit 9; increased expression occurs in response to ethanol consumption [RGD, Feb 2006]

Gene Symbol:   Atp5g1
Gene Name:   ATP synthase lipid-binding protein, mitochondrial
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000007235
Entrez:   29754
Chromosome Mapping:   10q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996300
y-intercept 37.350000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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