PrimePCR™ Probe Assay: Zfp384, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Zfp384, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Zfp384, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0037783
Assay Design:   intron_spanning
Chromosome Location:   4:224542268-224556565question
Amplicon Length:   182
Splice Variants Targeted:   ENSRNOT00000066923 ENSRNOT00000055985 ENSRNOT00000036654 ENSRNOT00000023240 ENSRNOT00000023192

Gene Information

activates transcription of matrix metalloproteinase genes; plays a role in nucleocytoplasmic transport [RGD, Feb 2006]

Gene Symbol:   Zfp384
Gene Name:   Zinc finger protein 384
Aliases:   Not Available
RefSeq:   NM_133429 NM_001034830 NM_001034831
Ensembl:   ENSRNOG00000017066
Entrez:   171018
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.010000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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