PrimePCR™ Probe Assay: Ube2f, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0032990
Assay Design:   intron_spanning
Chromosome Location:   9:98098437-98104833question
Amplicon Length:   182
Splice Variants Targeted:   ENSRNOT00000027050 ENSRNOT00000042082 ENSRNOT00000046864

Gene Information

Description Not Available

Gene Symbol:   Ube2f
Gene Name:   NEDD8-conjugating enzyme UBE2F
Aliases:   Not Available
RefSeq:   NM_001008381
Ensembl:   ENSRNOG00000019953
Entrez:   363284
UniGene:   Rn.62010
Chromosome Mapping:   9q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.910000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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