PrimePCR™ Probe Assay: Cenpc1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0031302
Assay Design:   intron_spanning
Chromosome Location:   14:23524474-23534030question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000031074

Gene Information

centromere autoantigen protein and a component of the inner kinetochore plate; essential for maintanence of kinetochore size and the timely transition to anaphase [RGD, Feb 2006]

Gene Symbol:   Cenpc1
Gene Name:   Centromere protein C 1
Aliases:   Not Available
RefSeq:   NM_001004098
Ensembl:   ENSRNOG00000021776
Entrez:   305270
UniGene:   Rn.85211
Chromosome Mapping:   14p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.130000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download