PrimePCR™ Probe Assay: Aqp4, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0030764
Assay Design:   intron_spanning
Chromosome Location:   18:6724972-6732199question
Amplicon Length:   172
Splice Variants Targeted:   ENSRNOT00000068150

Gene Information

The protein encoded by this gene forms a water-specific channel that regulates body water balance, particularly in the brain. The encoded protein acts as a membrane-bound homotetramer, interacting with other proteins (MLC1, TRPV4, HEPACAM, and ATP1B1) to form a complex. Several variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Gene Symbol:   Aqp4
Gene Name:   Aquaporin-4
Aliases:   Not Available
RefSeq:   NM_012825 NM_001142366
Ensembl:   ENSRNOG00000016043
Entrez:   25293
UniGene:   Rn.90091
Chromosome Mapping:   18p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download