PrimePCR™ Probe Assay: Tmed10, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0030568
Assay Design:   intron_spanning
Chromosome Location:   6:116373358-116381799question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000010512

Gene Information

membrane protein involved in vesicular protein trafficking; may be involved in vesicular transport from endoplasmic reticulum to the golgi [RGD, Feb 2006]

Gene Symbol:   Tmed10
Gene Name:   Transmembrane emp24 domain-containing protein 10
Aliases:   Not Available
RefSeq:   NM_053467
Ensembl:   ENSRNOG00000007901
Entrez:   84599
UniGene:   Rn.8509
Chromosome Mapping:   6q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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