PrimePCR™ Probe Assay: Cacng8, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cacng8, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cacng8, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0030016
Assay Design:   intron_spanning
Chromosome Location:   1:63322413-63325225question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000019300

Gene Information

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of the pore-forming alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma subunits. The protein encoded by this gene represents one of the gamma subunits. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is located in a cluster with two similar gamma subunit-encoding genes on chromosome 1. The mRNA for this gene is believed to initiate translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008]

Gene Symbol:   Cacng8
Gene Name:   Rno-mir-935
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000014348
Entrez:   140729
Chromosome Mapping:   1q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.230000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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