PrimePCR™ Probe Assay: Gucy2c, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029913
Assay Design:   intron_spanning
Chromosome Location:   4:234982732-234986470question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000012212

Gene Information

guanylyl cyclase; functions as receptor for heat-stable enterotoxin responsible for acute diarrhea [RGD, Feb 2006]

Gene Symbol:   Gucy2c
Gene Name:   Heat-stable enterotoxin receptor
Aliases:   Not Available
RefSeq:   NM_013170
Ensembl:   ENSRNOG00000009031
Entrez:   25711
UniGene:   Rn.10931
Chromosome Mapping:   4q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.430000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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