PrimePCR™ Probe Assay: Mitf, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029889
Assay Design:   intron_spanning
Chromosome Location:   4:194889598-194897161question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000051121

Gene Information

basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006]

Gene Symbol:   Mitf
Gene Name:   Microphthalmia-associated transcription factor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000008658
Entrez:   25094
UniGene:   Rn.31427
Chromosome Mapping:   4q34-q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.250000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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