PrimePCR™ Probe Assay: Klhl13, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029274
Assay Design:   intron_spanning
Chromosome Location:   X:121718368-121720575question
Amplicon Length:   105
Splice Variants Targeted:   ENSRNOT00000018788 ENSRNOT00000063868 ENSRNOT00000067114 ENSRNOT00000065459 ENSRNOT00000008510

Gene Information

Description Not Available

Gene Symbol:   Klhl13
Gene Name:   Protein Klhl13
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000014029
Entrez:   313445
UniGene:   Rn.24229
Chromosome Mapping:   Xq12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download