This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Kif5a, Rat
PrimePCR™ Template for Probe Assay: Kif5a, Rat
neuron-specific member of the kinesin 5 (Kif5) family, consisting of the heavy chains of conventional kinesins; may be a microtubule motor protein; mutation in human homolog causes autosomal dominant spastic paraplegia 10 [RGD, Feb 2006]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.