PrimePCR™ Probe Assay: Xdh, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029130
Assay Design:   intron_spanning
Chromosome Location:   6:35008227-35015079question
Amplicon Length:   75
Splice Variants Targeted:   ENSRNOT00000009634

Gene Information

This gene encodes a member of the xanthine dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein is involved in purine degradation and functions as both a dehydrogenase and an oxidase. [provided by RefSeq, Jan 2014]

Gene Symbol:   Xdh
Gene Name:   Xanthine dehydrogenase/oxidase
Aliases:   Not Available
RefSeq:   NM_017154
Ensembl:   ENSRNOG00000007081
Entrez:   497811
UniGene:   Rn.202951
Chromosome Mapping:   6q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.220000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download