PrimePCR™ Probe Assay: Gal, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029109
Assay Design:   intron_spanning
Chromosome Location:   1:225521143-225522527question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000020425

Gene Information

binds G-protein coupled receptors GalR1, GalR2, and GalR3; involved in negative regulation of immature thymocyte proliferation; plays a role in regulation of the immune system [RGD, Feb 2006]

Gene Symbol:   Gal
Gene Name:   Galanin peptides Galanin Galanin message-associated peptide
Aliases:   Not Available
RefSeq:   NM_033237
Ensembl:   ENSRNOG00000015156
Entrez:   29141
UniGene:   Rn.8929
Chromosome Mapping:   1q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.330000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download