PrimePCR™ Probe Assay: Pc, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029011
Assay Design:   intron_spanning
Chromosome Location:   1:226708536-226709896question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000026316

Gene Information

biotin-containing enzyme that catalyses the carboxylation of pyruvate to form oxaloacetate [RGD, Feb 2006]

Gene Symbol:   Pc
Gene Name:   Pyruvate carboxylase, mitochondrial
Aliases:   Not Available
RefSeq:   NM_012744
Ensembl:   ENSRNOG00000019372
Entrez:   25104
UniGene:   Rn.11094
Chromosome Mapping:   1q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.590000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download