PrimePCR™ Probe Assay: Gstm2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028952
Assay Design:   intron_spanning
Chromosome Location:   2:230247309-230249236question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000025939

Gene Information

subunit of glutathione S-transferase, which may play a role in xenobiotic metabolism and response to oxidative stress [RGD, Feb 2006]

Gene Symbol:   Gstm2
Gene Name:   Glutathione S-transferase Mu 2
Aliases:   Not Available
RefSeq:   NM_177426
Ensembl:   ENSRNOG00000019094
Entrez:   24424
UniGene:   Rn.625
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.800000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download