PrimePCR™ Probe Assay: Ncstn, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028735
Assay Design:   intron_spanning
Chromosome Location:   13:94978780-94980008question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000008150

Gene Information

component of the gamma-secretase complex, which catalyzes the cleavage of amyloid precursor protein APP [RGD, Feb 2006]

Gene Symbol:   Ncstn
Gene Name:   Nicastrin
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000005355
Entrez:   289231
UniGene:   Rn.51975
Chromosome Mapping:   13q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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