PrimePCR™ Probe Assay: Gtf2i, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028687
Assay Design:   intron_spanning
Chromosome Location:   12:27474740-27479745question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000023330 ENSRNOT00000002020 ENSRNOT00000040356

Gene Information

Description Not Available

Gene Symbol:   Gtf2i
Gene Name:   General transcription factor II-I
Aliases:   Not Available
RefSeq:   NM_001001512
Ensembl:   ENSRNOG00000001479
Entrez:   353256
UniGene:   Rn.27575
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.820000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download