PrimePCR™ Probe Assay: Msra, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028519
Assay Design:   intron_spanning
Chromosome Location:   15:51400541-51463864question
Amplicon Length:   153
Splice Variants Targeted:   ENSRNOT00000072997 ENSRNOT00000016616 ENSRNOT00000071197 ENSRNOT00000022814 ENSRNOT00000037397

Gene Information

catalyzes the reduction of methionine sulphoxide to methionine in proteins [RGD, Feb 2006]

Gene Symbol:   Msra
Gene Name:   Mitochondrial peptide methionine sulfoxide reductase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012440
Entrez:   29447
UniGene:   Rn.163306
Chromosome Mapping:   15p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.530000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download