PrimePCR™ Probe Assay: Tnfrsf1b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028495
Assay Design:   intron_spanning
Chromosome Location:   5:166821288-166824656question
Amplicon Length:   86
Splice Variants Targeted:   ENSRNOT00000022478

Gene Information

mediates brain uptake of tumor necrosis factor (TNF)-alpha from the circulation [RGD, Feb 2006]

Gene Symbol:   Tnfrsf1b
Gene Name:   Tumor necrosis factor receptor superfamily member 1B
Aliases:   Not Available
RefSeq:   NM_130426
Ensembl:   ENSRNOG00000016575
Entrez:   156767
UniGene:   Rn.83633
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.450000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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