PrimePCR™ Probe Assay: Limk2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028480
Assay Design:   intron_spanning
Chromosome Location:   14:84276083-84277969question
Amplicon Length:   135
Splice Variants Targeted:   ENSRNOT00000004901 ENSRNOT00000026032 ENSRNOT00000050005 ENSRNOT00000057673

Gene Information

kinase with a LIM-kinase (LIMK) domain; human homolog phosphorylates cofilin [RGD, Feb 2006]

Gene Symbol:   Limk2
Gene Name:   LIM domain kinase 2
Aliases:   Not Available
RefSeq:   NM_024135
Ensembl:   ENSRNOG00000019000
Entrez:   29524
UniGene:   Rn.11013
Chromosome Mapping:   14q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.960000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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