PrimePCR™ Probe Assay: Slc33a1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028386
Assay Design:   intron_spanning
Chromosome Location:   2:173904985-173906267question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000013392

Gene Information

acetyl CoA transporter involved in ganglioside O-acetylation; may function in embryonic development of the nervous system [RGD, Feb 2006]

Gene Symbol:   Slc33a1
Gene Name:   Acetyl-coenzyme A transporter 1
Aliases:   Not Available
RefSeq:   NM_022252
Ensembl:   ENSRNOG00000010023
Entrez:   64018
UniGene:   Rn.209601
Chromosome Mapping:   2q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.030000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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