PrimePCR™ Probe Assay: Panx1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028306
Assay Design:   intron_spanning
Chromosome Location:   8:13509122-13510377question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000013577

Gene Information

The protein encoded by this gene is a plasma membrane protein that is a structural component of gap junctions. The encoded protein acts as a homodimer or as a heterodimer with other isoforms or proteins. Two additional variants have been found, and the isoforms expressed from them are found in the cytoplasm. It is thought that these two isoforms could attenuate the actions of the membrane-bound protein. [provided by RefSeq, Jul 2012]

Gene Symbol:   Panx1
Gene Name:   Pannexin-1
Aliases:   Not Available
RefSeq:   NM_199397
Ensembl:   ENSRNOG00000010060
Entrez:   315435
UniGene:   Rn.204521
Chromosome Mapping:   8q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download