This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Panx1, Rat
PrimePCR™ Template for Probe Assay: Panx1, Rat
The protein encoded by this gene is a plasma membrane protein that is a structural component of gap junctions. The encoded protein acts as a homodimer or as a heterodimer with other isoforms or proteins. Two additional variants have been found, and the isoforms expressed from them are found in the cytoplasm. It is thought that these two isoforms could attenuate the actions of the membrane-bound protein. [provided by RefSeq, Jul 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.