PrimePCR™ Probe Assay: Fah, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028258
Assay Design:   intron_spanning
Chromosome Location:   1:147658313-147659368question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000068167

Gene Information

deficiency leads to human hereditary tyrosinemia type 1 [RGD, Feb 2006]

Gene Symbol:   Fah
Gene Name:   Fumarylacetoacetase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000013223
Entrez:   29383
UniGene:   Rn.9195
Chromosome Mapping:   1q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.610000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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