PrimePCR™ Probe Assay: Abcg1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0027987
Assay Design:   intron_spanning
Chromosome Location:   20:12017902-12019082question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000001533

Gene Information

ATP-binding cassette (ABC) half-transporter that facilitates the efflux of excess cholesterol from macrophages and may play an important role in cholesterol homeostasis [RGD, Feb 2006]

Gene Symbol:   Abcg1
Gene Name:   ATP-binding cassette sub-family G member 1
Aliases:   Not Available
RefSeq:   NM_053502
Ensembl:   ENSRNOG00000001158
Entrez:   85264
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.400000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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