PrimePCR™ Probe Assay: Robo2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026271
Assay Design:   intron_spanning
Chromosome Location:   11:14882998-14884040question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000065748

Gene Information

repulsive axon guidance receptor for the slit protein ligands; plays a role in axon guidance and repulsion [RGD, Feb 2006]

Gene Symbol:   Robo2
Gene Name:   Roundabout 2 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029598
Entrez:   84409
UniGene:   Rn.24350,Rn.214590
Chromosome Mapping:   11p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.270000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download