PrimePCR™ Probe Assay: Gucy1a3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026255
Assay Design:   intron_spanning
Chromosome Location:   2:200475401-200480978question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000017190

Gene Information

soluble guanylate cyclase subunit that forms a heterodimer with Gucy1b3; may also function as an intracellular nitric oxide receptor [RGD, Feb 2006]

Gene Symbol:   Gucy1a3
Gene Name:   Guanylate cyclase soluble subunit alpha-3
Aliases:   Not Available
RefSeq:   NM_017090
Ensembl:   ENSRNOG00000012302
Entrez:   497757
Chromosome Mapping:   2q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.300000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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