PrimePCR™ Probe Assay: Plin2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025823
Assay Design:   intron_spanning
Chromosome Location:   5:108988925-108991018question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000009749

Gene Information

intrinsic lipid storage droplet protein; involved in transfer of lipid between lipofibroblasts and alveolar type II epithelial cells [RGD, Feb 2006]

Gene Symbol:   Plin2
Gene Name:   Perilipin-2
Aliases:   Not Available
RefSeq:   NM_001007144
Ensembl:   ENSRNOG00000007060
Entrez:   298199
UniGene:   Rn.101967
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.180000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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