PrimePCR™ Probe Assay: Gtf2b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025139
Assay Design:   intron_spanning
Chromosome Location:   2:267255899-267257532question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000015032

Gene Information

putative subunit of RNA polymerase II [RGD, Feb 2006]

Gene Symbol:   Gtf2b
Gene Name:   Transcription initiation factor IIB
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000011135
Entrez:   81673
UniGene:   Rn.6109
Chromosome Mapping:   2q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.840000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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