PrimePCR™ Probe Assay: Erap1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024740
Assay Design:   intron_spanning
Chromosome Location:   2:1394768-1396747question
Amplicon Length:   92
Splice Variants Targeted:   ENSRNOT00000013625

Gene Information

aminopeptidase enzyme; catalyzes the puromycin-insensitive Zn-dependent hydrolysis of N-terminal Leu; plays a role in processing antigenic precursors for MHC class I presentation [RGD, Feb 2006]

Gene Symbol:   Erap1
Gene Name:   Endoplasmic reticulum aminopeptidase 1 precursor
Aliases:   Not Available
RefSeq:   NM_030836
Ensembl:   ENSRNOG00000009997
Entrez:   80897
UniGene:   Rn.22516
Chromosome Mapping:   2q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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