PrimePCR™ Probe Assay: Aplp1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024736
Assay Design:   intron_spanning
Chromosome Location:   1:90054395-90056602question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000028297

Gene Information

interacts with Fe65 and Fe65L2, members of a family of proteins that bind beta-amyloid precursor protein [RGD, Feb 2006]

Gene Symbol:   Aplp1
Gene Name:   Amyloid-like protein 1 precursor
Aliases:   Not Available
RefSeq:   NM_001100802
Ensembl:   ENSRNOG00000020851
Entrez:   502317
UniGene:   Rn.114169
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.030000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download