PrimePCR™ Probe Assay: Hnrnpa2b1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hnrnpa2b1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hnrnpa2b1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024454
Assay Design:   intron_spanning
Chromosome Location:   4:145903691-145904771question
Amplicon Length:   189
Splice Variants Targeted:   ENSRNOT00000070930 ENSRNOT00000071214 ENSRNOT00000067921 ENSRNOT00000068563 ENSRNOT00000066077 ENSRNOT00000022497 ENSRNOT00000047241 ENSRNOT00000001912 ENSRNOT00000003928 ENSRNOT00000003869 ENSRNOT00000060977 ENSRNOT00000074424 ENSRNOT00000050179 ENSRNOT00000013960 ENSRNOT00000072743 ENSRNOT00000015152 ENSRNOT00000032785 ENSRNOT00000075678 ENSRNOT00000009173 ENSRNOT00000002929 ENSRNOT00000011567 ENSRNOT00000049492 ENSRNOT00000038067 ENSRNOT00000024353 ENSRNOT00000073685

Gene Information

Description Not Available

Gene Symbol:   Hnrnpa2b1
Gene Name:   Heterogeneous nuclear ribonucleoproteins A2/B1
Aliases:   Not Available
RefSeq:   NM_001104613
Ensembl:   ENSRNOG00000011175
Entrez:   362361
Chromosome Mapping:   4q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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