PrimePCR™ Probe Assay: Cttn, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024325
Assay Design:   intron_spanning
Chromosome Location:   1:224463488-224464639question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000023861 ENSRNOT00000033827 ENSRNOT00000033919 ENSRNOT00000041034 ENSRNOT00000011290 ENSRNOT00000059974 ENSRNOT00000041988 ENSRNOT00000011371 ENSRNOT00000071529 ENSRNOT00000066774 ENSRNOT00000020768 ENSRNOT00000010895 ENSRNOT00000051590 ENSRNOT00000040845 ENSRNOT00000030344 ENSRNOT00000015181 ENSRNOT00000026448 ENSRNOT00000030037 ENSRNOT00000027836 ENSRNOT00000028283 ENSRNOT00000047669 ENSRNOT00000054859 ENSRNOT00000020763

Gene Information

component of clathrin-coated pits; involved in receptor-mediated endocytosis [RGD, Feb 2006]

Gene Symbol:   Cttn
Gene Name:   Cortactin; Cortactin, isoform CRA_e; Protein Cttn
Aliases:   Not Available
RefSeq:   NM_021868
Ensembl:   ENSRNOG00000047280
Entrez:   60465
UniGene:   Rn.107869
Chromosome Mapping:   1q41-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 41.310000
Efficiency 82

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download