PrimePCR™ Probe Assay: Igf2bp2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Igf2bp2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Igf2bp2, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023875
Assay Design:   intron_spanning
Chromosome Location:   11:85708892-85709956question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000036958

Gene Information

The protein encoded by this gene is a member of a family of mRNA binding proteins that have been shown to bind to several different mRNAs in mammalian cells, including insulin-like growth factor 2 mRNA. Generation of two different isoforms by alternative translation initiation at an internal start codon, has been described for this gene (PMID:22427968). [provided by RefSeq, Jul 2012]

Gene Symbol:   Igf2bp2
Gene Name:   Insulin-like growth factor 2 mRNA-binding protein 2 isoform 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000025946
Entrez:   303824
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.630000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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