PrimePCR™ Probe Assay: Rpl19, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023028
Assay Design:   intron_spanning
Chromosome Location:   10:85768073-85773741question
Amplicon Length:   177
Splice Variants Targeted:   ENSRNOT00000056688 ENSRNOT00000075190 ENSRNOT00000025824 ENSRNOT00000073339 ENSRNOT00000006359 ENSRNOT00000027086 ENSRNOT00000071025 ENSRNOT00000072567 ENSRNOT00000075802 ENSRNOT00000007683 ENSRNOT00000059158

Gene Information

ribosomal protein [RGD, Feb 2006]

Gene Symbol:   Rpl19
Gene Name:   60S ribosomal protein L19
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000004741
Entrez:   81767
UniGene:   Rn.3384
Chromosome Mapping:   10q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download