PrimePCR™ Probe Assay: Eif4g1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0022911
Assay Design:   intron_spanning
Chromosome Location:   11:86980162-86982966question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000003274 ENSRNOT00000075306 ENSRNOT00000041777 ENSRNOT00000040029 ENSRNOT00000003593

Gene Information

Description Not Available

Gene Symbol:   Eif4g1
Gene Name:   Protein Eif4g1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001738
Entrez:   287986
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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