PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048720, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048720, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048720, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0054975
Assay Design:   Intron-spanning
Chromosome Location:   19:37043266-37049318question
Amplicon Length:   86
Splice Variants Targeted:   ENSRNOT00000021769 ENSRNOT00000050902 ENSRNOT00000072324 ENSRNOT00000029985 ENSRNOT00000011438 ENSRNOT00000061446 ENSRNOT00000072065 ENSRNOT00000066121 ENSRNOT00000017005 ENSRNOT00000072950 ENSRNOT00000002427 ENSRNOT00000008680 ENSRNOT00000072788 ENSRNOT00000032723 ENSRNOT00000021271 ENSRNOT00000022022 ENSRNOT00000023709 ENSRNOT00000038953 ENSRNOT00000011855 ENSRNOT00000022659 ENSRNOT00000022705 ENSRNOT00000006843 ENSRNOT00000073834 ENSRNOT00000022483 ENSRNOT00000021685 ENSRNOT00000010714 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048720
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048720
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.490000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download