PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048269, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048269, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048269, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0054916
Assay Design:   Intron-spanning
Chromosome Location:   1:205143706-205152664question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000072324 ENSRNOT00000029985 ENSRNOT00000026413 ENSRNOT00000061446 ENSRNOT00000072065 ENSRNOT00000019072 ENSRNOT00000017005 ENSRNOT00000036876 ENSRNOT00000026195 ENSRNOT00000072788 ENSRNOT00000022022 ENSRNOT00000011855 ENSRNOT00000012222 ENSRNOT00000030244 ENSRNOT00000034264 ENSRNOT00000057830 ENSRNOT00000057218 ENSRNOT00000006752 ENSRNOT00000001536 ENSRNOT00000010753 ENSRNOT00000075110 ENSRNOT00000010229 ENSRNOT00000023226 ENSRNOT00000068334 ENSRNOT00000038612 ENSRNOT00000071526 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048269
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048269
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download