PrimePCR™ SYBR® Green Assay: Cacna1i, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0054324
Assay Design:   Intron-spanning
Chromosome Location:   7:121575377-121581644question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000048790

Gene Information

low voltage-activated calcium channel subunit; important for calcium ion transport into nerve, muscle and endocrine cells [RGD, Feb 2006]

Gene Symbol:   Cacna1i
Gene Name:   Voltage-dependent T-type calcium channel subunit alpha-1I
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029914
Entrez:   56827
UniGene:   Rn.48680
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.880000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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