PrimePCR™ SYBR® Green Assay: Hfe, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0051623
Assay Design:   Intron-spanning
Chromosome Location:   17:45521365-45523027question
Amplicon Length:   168
Splice Variants Targeted:   ENSRNOT00000010050 ENSRNOT00000002321 ENSRNOT00000022881 ENSRNOT00000022809 ENSRNOT00000022810 ENSRNOT00000016555 ENSRNOT00000005685 ENSRNOT00000016380 ENSRNOT00000064438 ENSRNOT00000026792 ENSRNOT00000050763

Gene Information

membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006]

Gene Symbol:   Hfe
Gene Name:   Hereditary hemochromatosis protein homolog
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000016967
Entrez:   29199
UniGene:   Rn.42931
Chromosome Mapping:   17p11-q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 37.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download