PrimePCR™ SYBR® Green Assay: Slc7a9, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0051538
Assay Design:   Intron-spanning
Chromosome Location:   1:92850229-92851320question
Amplicon Length:   116
Splice Variants Targeted:   ENSRNOT00000016919

Gene Information

amino acid transporter protein; exhibits high-affinity sodium-independent transport of cystine; may mediate reabsorption of cystine in the kidney proximal tubule [RGD, Feb 2006]

Gene Symbol:   Slc7a9
Gene Name:   B(0,+)-type amino acid transporter 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012344
Entrez:   116726
UniGene:   Rn.32225
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.993400
y-intercept 37.330000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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