PrimePCR™ SYBR® Green Assay: RGD1563169, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RGD1563169, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1563169, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0051275
Assay Design:   Intron-spanning
Chromosome Location:   10:41367289-41368220question
Amplicon Length:   124
Splice Variants Targeted:   ENSRNOT00000014546 ENSRNOT00000001783 ENSRNOT00000004602 ENSRNOT00000046847 ENSRNOT00000003565 ENSRNOT00000068337 ENSRNOT00000039210 ENSRNOT00000020414 ENSRNOT00000066877 ENSRNOT00000059993 ENSRNOT00000058627 ENSRNOT00000024794 ENSRNOT00000016784 ENSRNOT00000049793 ENSRNOT00000027271 ENSRNOT00000003122 ENSRNOT00000057929 ENSRNOT00000065393 ENSRNOT00000068459 ENSRNOT00000024803 ENSRNOT00000009043

Gene Information

Description Not Available

Gene Symbol:   RGD1563169
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038474
Entrez:   363602
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.810000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download