PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000020154, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0022172
Assay Design:   intron_spanning
Chromosome Location:   1:222232489-222319379question
Amplicon Length:   153
Splice Variants Targeted:   ENSRNOT00000002292 ENSRNOT00000034940 ENSRNOT00000008055 ENSRNOT00000031549 ENSRNOT00000024161 ENSRNOT00000073221 ENSRNOT00000013577 ENSRNOT00000070993 ENSRNOT00000051307 ENSRNOT00000064212 ENSRNOT00000035996 ENSRNOT00000025948 ENSRNOT00000049064 ENSRNOT00000035541 ENSRNOT00000017112 ENSRNOT00000061735 ENSRNOT00000015092 ENSRNOT00000001826 ENSRNOT00000045827 ENSRNOT00000005352 ENSRNOT00000008184 ENSRNOT00000056735 ENSRNOT00000038972 ENSRNOT00000071031 ENSRNOT00000045400 ENSRNOT00000068102 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000020154
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000020154
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.990000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download