PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046498, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000046498, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000046498, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0021428
Assay Design:   intron_spanning
Chromosome Location:   7:1378934-1380913question
Amplicon Length:   153
Splice Variants Targeted:   ENSRNOT00000043153 ENSRNOT00000061525 ENSRNOT00000049746 ENSRNOT00000051066 ENSRNOT00000073775 ENSRNOT00000071982 ENSRNOT00000043269 ENSRNOT00000046852 ENSRNOT00000073673 ENSRNOT00000074599 ENSRNOT00000071620 ENSRNOT00000073828 ENSRNOT00000051488 ENSRNOT00000073169 ENSRNOT00000074550 ENSRNOT00000048862 ENSRNOT00000050485 ENSRNOT00000075578 ENSRNOT00000038077 ENSRNOT00000072533 ENSRNOT00000048256

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046498
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046498
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download