PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000047540, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000047540, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000047540, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0020654
Assay Design:   intron_spanning
Chromosome Location:   1:70648683-70650211question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000021263 ENSRNOT00000066583 ENSRNOT00000024704 ENSRNOT00000064347 ENSRNOT00000028786 ENSRNOT00000058616 ENSRNOT00000007110 ENSRNOT00000072183 ENSRNOT00000001750 ENSRNOT00000016415 ENSRNOT00000001081 ENSRNOT00000007940 ENSRNOT00000016423 ENSRNOT00000033222 ENSRNOT00000058419 ENSRNOT00000016479 ENSRNOT00000018185 ENSRNOT00000016628 ENSRNOT00000019096 ENSRNOT00000037177 ENSRNOT00000005942 ENSRNOT00000073066 ENSRNOT00000056014

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000047540
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047540
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download