PrimePCR™ SYBR® Green Assay: Mcfd2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0019812
Assay Design:   intron_spanning
Chromosome Location:   6:20879178-20887267question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000020328

Gene Information

a survival factor involved in maintaining stem cell potential and lifelong neurogenesis in the adult central nervous system [RGD, Feb 2006]

Gene Symbol:   Mcfd2
Gene Name:   Multiple coagulation factor deficiency protein 2 homolog precursor
Aliases:   Not Available
RefSeq:   NM_139253
Ensembl:   ENSRNOG00000015059
Entrez:   246117
UniGene:   Rn.7871
Chromosome Mapping:   6q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.210000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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