PrimePCR™ SYBR® Green Assay: Tecr, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0018551
Assay Design:   intron_spanning
Chromosome Location:   19:35687005-35711341question
Amplicon Length:   182
Splice Variants Targeted:   ENSRNOT00000037608

Gene Information

synaptic glycoprotein expressed in many types of neurons [RGD, Feb 2006]

Gene Symbol:   Tecr
Gene Name:   Trans-2,3-enoyl-CoA reductase
Aliases:   Not Available
RefSeq:   NM_138549
Ensembl:   ENSRNOG00000021808
Entrez:   191576
UniGene:   Rn.107358
Chromosome Mapping:   19q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 37.330000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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