PrimePCR™ SYBR® Green Assay: Oas1d, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0018351
Assay Design:   intron_spanning
Chromosome Location:   12:44222031-44224513question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000002289 ENSRNOT00000041025 ENSRNOT00000043971 ENSRNOT00000045699 ENSRNOT00000060194 ENSRNOT00000052018 ENSRNOT00000000535 ENSRNOT00000073363 ENSRNOT00000019894 ENSRNOT00000011630 ENSRNOT00000025878 ENSRNOT00000043215 ENSRNOT00000031134 ENSRNOT00000067135 ENSRNOT00000037570 ENSRNOT00000041741 ENSRNOT00000034036 ENSRNOT00000067176 ENSRNOT00000057279 ENSRNOT00000038426 ENSRNOT00000048450 ENSRNOT00000067387 ENSRNOT00000001851 ENSRNOT00000001848 ENSRNOT00000001853 ENSRNOT00000057266 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Oas1d
Gene Name:   Protein Oas1d
Aliases:   Not Available
RefSeq:   NM_001009379
Ensembl:   ENSRNOG00000048485
Entrez:   304508
UniGene:   Rn.110155
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.720000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download