PrimePCR™ SYBR® Green Assay: Psgb1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0018141
Assay Design:   intron_spanning
Chromosome Location:   1:80431620-80434542question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000037132

Gene Information

putative pregnancy-specific glycoprotein (Psg); human homolog contributes to immunomodulation during pregnancy [RGD, Feb 2006]

Gene Symbol:   Psgb1
Gene Name:   Pregnancy-specific beta 1-glycoprotein
Aliases:   Not Available
RefSeq:   NM_021677
Ensembl:   ENSRNOG00000027288
Entrez:   59313
UniGene:   Rn.42878
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.230000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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