PrimePCR™ SYBR® Green Assay: Gtpbp6, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gtpbp6, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gtpbp6, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0016794
Assay Design:   intron_spanning
Chromosome Location:   12:54374142-54375052question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000061883 ENSRNOT00000014887 ENSRNOT00000061516 ENSRNOT00000061515 ENSRNOT00000061513 ENSRNOT00000049292 ENSRNOT00000002324 ENSRNOT00000016340 ENSRNOT00000026819 ENSRNOT00000045256 ENSRNOT00000001602 ENSRNOT00000001912 ENSRNOT00000064070 ENSRNOT00000065431 ENSRNOT00000007640 ENSRNOT00000070873 ENSRNOT00000031190 ENSRNOT00000058496 ENSRNOT00000005541 ENSRNOT00000044413 ENSRNOT00000063905 ENSRNOT00000056632 ENSRNOT00000026941 ENSRNOT00000022849 ENSRNOT00000043082 ENSRNOT00000068394 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Gtpbp6
Gene Name:   Protein Gtpbp6
Aliases:   Not Available
RefSeq:   NM_001135840
Ensembl:   ENSRNOG00000048168
Entrez:   363931
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download